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nexterion slide h microarray slides  (SCHOTT)

 
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    SCHOTT nexterion slide h microarray slides
    Nexterion Slide H Microarray Slides, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nexterion+slide+h+microarray+slides/nexterion+slide+h+microarray+slides/pmc11615817-469-0-8
    Average 90 stars, based on 1 article reviews
    nexterion slide h microarray slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Micromotion Derived Fluid Shear Stress Mediates Peri‐Electrode Gliosis through Mechanosensitive Ion Channels
    Article Snippet: .. Nexterion slide H microarray slides were acquired from Schott AG (Mainz, Germany). .. CF 555, succinimidyl ester was purchased from Sigma‐Aldrich (SCJ4600022, Dublin, Ireland).

    Article Title: Self-powered piezo-bioelectronic device mediates tendon repair through modulation of mechanosensitive ion channels
    Article Snippet: .. Nexterion slide H microarray slides were purchased from Schott AG (Mainz, Germany). .. Alexa Fluor 555 carboxylic acid succinimidyl ester was obtained from Life Technologies (Carlsbad, CA, USA).

    Article Title: A Tympanic Piezo‐Bioreactor Modulates Ion Channel‐Associated Mechanosignaling to Stabilize Phenotype and Promote Tenogenesis in Human Tendon‐Derived Cells.
    Article Snippet: .. Nexterion slide H microarray slides were purchased from Schott AG (Mainz, Germany). .. Alexa Fluor 555 carboxylic acid succinimidyl ester was obtained from Life Technologies (Carlsbad, CA, USA).

    Article Title: Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A
    Article Snippet: The rabbit polyclonal antiglial fibrillary acidic protein (GFAP) antibody was from DakoCytomation (Dublin, Ireland), the rabbit polyclonal antineurogranin antibody from Abcam (Cambridge, U.K.), and the mouse monoclonal anti-βI-integrin antibody were from Chemicon International Inc. (Temecula, CA). .. Nexterion Slide H microarray slides were supplied by Schott AG (Mainz, Germany). .. The 8-well gasket slide and incubation cassette system was from Agilent Technologies Ireland, Ltd. (Cork, Ireland).

    Article Title: Smac mimetic suppresses tunicamycin-induced apoptosis via resolution of ER stress
    Article Snippet: The Mem-PER Eukaryotic Membrane Protein Extraction Reagent Kit, protease inhibitor cocktail (EDTA-free), and Alexa Fluor ® 555 NHS ester were purchased from Thermo Fisher Scientific Inc. (Dublin, Ireland). .. Centrifugal Ultracel ® low binding regenerated cellulose filters (0.5 ml, 3 kDa molecular weight cut-off (MWCO)) were obtained from Merck Millipore (Cork, Ireland), and the Nexterion ® slide H microarray slides from Schott AG (Mainz, Germany). .. Apoptosis was determined by analysis of DNA fragmentation of propidium iodide (PI)-stained nuclei using flow cytometry (FACSCanto II, BD Biosciences, Heidelberg, Germany) as described previously .

    Article Title: A Self-Powered Piezo-Bioelectric Device Regulates Tendon Repair-Associated Signaling Pathways through Modulation of Mechanosensitive Ion Channels.
    Article Snippet: .. Nexterion slide H microarray slides were purchased from Schott AG (Mainz, Germany). .. Alexa Fluor 555 carboxylic acid succinimidyl ester was obtained from Life Technologies (Carlsbad, CA, USA).

    Article Title: Micromotion derived fluid shear stress mediates peri-electrode gliosis through mechanosensitive ion channels
    Article Snippet: .. Nexterion slide H microarray slides were acquired from Schott AG (Mainz, Germany). .. CFTM 555, succinimidyl ester was purchased from Sigma-Aldrich® (SCJ4600022, Dublin, Ireland).

    Article Title: Micromotion Derived Fluid Shear Stress Mediates Peri-Electrode Gliosis through Mechanosensitive Ion Channels.
    Article Snippet: .. Protein Antibody Microarray: Nexterion slide H microarray slides were acquired from Schott AG (Mainz, Germany). .. CF 555, succinimidyl ester was purchased from Sigma-Aldrich (SCJ4600022, Dublin, Ireland).

    Binding Assay:

    Article Title: Smac mimetic suppresses tunicamycin-induced apoptosis via resolution of ER stress
    Article Snippet: The Mem-PER Eukaryotic Membrane Protein Extraction Reagent Kit, protease inhibitor cocktail (EDTA-free), and Alexa Fluor ® 555 NHS ester were purchased from Thermo Fisher Scientific Inc. (Dublin, Ireland). .. Centrifugal Ultracel ® low binding regenerated cellulose filters (0.5 ml, 3 kDa molecular weight cut-off (MWCO)) were obtained from Merck Millipore (Cork, Ireland), and the Nexterion ® slide H microarray slides from Schott AG (Mainz, Germany). .. Apoptosis was determined by analysis of DNA fragmentation of propidium iodide (PI)-stained nuclei using flow cytometry (FACSCanto II, BD Biosciences, Heidelberg, Germany) as described previously .

    Molecular Weight:

    Article Title: Smac mimetic suppresses tunicamycin-induced apoptosis via resolution of ER stress
    Article Snippet: The Mem-PER Eukaryotic Membrane Protein Extraction Reagent Kit, protease inhibitor cocktail (EDTA-free), and Alexa Fluor ® 555 NHS ester were purchased from Thermo Fisher Scientific Inc. (Dublin, Ireland). .. Centrifugal Ultracel ® low binding regenerated cellulose filters (0.5 ml, 3 kDa molecular weight cut-off (MWCO)) were obtained from Merck Millipore (Cork, Ireland), and the Nexterion ® slide H microarray slides from Schott AG (Mainz, Germany). .. Apoptosis was determined by analysis of DNA fragmentation of propidium iodide (PI)-stained nuclei using flow cytometry (FACSCanto II, BD Biosciences, Heidelberg, Germany) as described previously .



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    nexterion® h microarray slides  (SCIENION)
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    Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) <t> microarray, </t> the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.
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    Image Search Results


    Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

    Journal: PLOS ONE

    Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

    doi: 10.1371/journal.pone.0283537

    Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

    Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

    Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

    (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

    Journal: PLOS ONE

    Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

    doi: 10.1371/journal.pone.0283537

    Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

    Article Snippet: All protein antigens in PBS, pH 7.4 ( ) were printed on Nexterion® H microarray slides in six replicate features, approximately 1 nL per feature (2 drops), with 8 replicate subarrays per microarray slide using a Scienion SciFlexArrayer S3 essentially as previously described [ ].

    Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining

    Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

    Journal: PLOS ONE

    Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

    doi: 10.1371/journal.pone.0283537

    Figure Lengend Snippet: Recombinant SARS-CoV-2 proteins used to construct the antigen (Ag) microarray, the Ag code, molecular mass (Mr) observed by SDS-PAGE, expression system, protein sequence expressed, supplier, catalogue number, and approximate print concentration (mg/mL). MPL, Molecular Parasitology Lab; RB, RayBiotech; R&D, R&D Systems. * Ag failed to conjugate and omitted from data.

    Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

    Techniques: Recombinant, Construct, Microarray, Expressing, Sequencing, Concentration Assay, Binding Assay, Membrane

    (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

    Journal: PLOS ONE

    Article Title: A new multiplex SARS-CoV-2 antigen microarray showed correlation of IgG, IgA, and IgM antibodies from patients with COVID-19 disease severity and maintenance of relative IgA and IgM antigen binding over time

    doi: 10.1371/journal.pone.0283537

    Figure Lengend Snippet: (A) Cartoon of SARS-CoV-2 protein antigens conjugated to a microarray surface in replicate subarray format. Each subarray was incubated with a purified serum antibody isotype and serum antibody binding to specific antigens was detected using fluorescently labelled anti-isotype antibodies. (B) Cartoon of a typical SARS-CoV-2 antigen microarray slide with six replicate subarrays. (C) Bar charts representing the binding intensity of diluted serum (1/100 in PBS-T) and purified serum (C) IgG, (D) IgA, and (E) IgM from a COVID-19 patient (203-0018-1) detected by the appropriate fluorescently-labelled anti-isotype antibody. (F) Flow chart depicting sequential serum immunoglobulin isotype purification procedure. (G) Purified serum IgG, IgA, and IgM (1 μg each) from a COVID-19 patient (203–0025) electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel and silver stained. L, molecular mass ladder (kDa).

    Article Snippet: Nexterion® slide H microarray slides were supplied by Schott AG (Mainz, Germany).

    Techniques: Microarray, Incubation, Purification, Binding Assay, SDS Page, Staining